How Can Karyotype Analysis Explain Genetic Disorders?

Introduction:
A karyotype is a picture of an organism's genetic make-up in which the chromosomes of a cell have been stained so that the banding pattern of the chromosomes appear.  Cells in Metaphase are stained to show distinct parts of the chromosomes.  The cells are then photographed through a microscope and enlarged.  The chromosomes are cut from the photograph and arranged according to size, shape, centromere position, and banding patterns.  Karyotypes have become of increasing importance to genetic counselors as disorders and diseases have been traced to specific visible abnormalities of the chromosome.

Objectives:
    1.  Construct a karyotype form the metaphase chromosomes of a fictitious organism
    2.  Analyze prepared karyotypes for chromosome abnormalities
    3.  Identify the genetic disorders of six fictitious cows by using the cows' karyotypes
    4.  Hypothesize how karyotypes analysis can be used to explain the presence of a genetic disorder

Materials:
    print-outs of the the metaphase chromosomes from six cows (2 pages)
    scissors
    glue

procedure

 

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This activity is based on a lab from Biology: Dynamics of Life Laboratory Manual, Glencoe, 1995.